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A cell culture method for studying transport across the intestinal barrier was investigated using a culture-based microsampling approach. Caco-2 cells grown on either permeable or impermeable support were used to develop a cell culture medium for sampling a central solution or collecting media from a peripheral reservoir solution. This was done by adding the cells to a new surface support in the center of a well, which was incubated with the cells for five days until confluency. This resulted in a culture of monolayers of Caco-2 cells on the inner surface of a permeable membrane and cultured cell media from a separate cell culture on the top surface of the membrane. Permeability studies were conducted on the two different forms of permeable membrane to measure the permeability coefficients to glucose and fluorescein. The permeability of Caco-2 cell cultures across the permeable membrane