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Previous experiments demonstrated that chronic hyperventilation can increase skeletal muscle glycogen, protein, triglyceride and calcium levels. In the present investigation, skeletal muscle glycogen and protein levels in four chronic metabolic acidosis rats (CMA) were compared with those in four control rats (CONT). The glycogen levels were increased significantly in both soleus and tibialis anterior muscles of the CMA rats at the end of the 1st h of a 6-h recovery period. In contrast, the protein levels were not significantly different in either muscle of the CMA or CONT rats at any of the times studied. Although the average values of pHi were not different between the groups, the pHi was actually lower in the soleus muscles of the CMA than in the CONT rats. This resulted in a higher average CO2 tension in the CMA than in the CONT rats throughout the 6-h recovery period. This suggests that the soleus muscles of the CMA rats were experiencing more severe metabolic acidosis than the CONT rats. The muscle CO2 tension was directly correlated with the average muscle glycogen levels (r = 0.85). The results of this experiment suggest that chronic metabolic acidosis results in skeletal muscle glycogen and CO2 retention which may cause muscle weakness.Q:
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