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LeseMeldungenIn vivo precision of 14C-acetate biodistribution studies in mice.
The precision and variability of (14)C-acetate biodistribution studies were determined in mice using two specific radioassays for the quantification of (14)C-acetate-derived carbon dioxide (CO(2)) and label in whole blood. (14)C-labeled acetate (5, 10, or 15 min) was administered as single i.v. bolus injections (120 kBq kg(-1)), five times at 5-day intervals, and the (14)C-carbon dioxide and (14)C-label were measured separately at various time intervals from 10 min to 3 months after injection. Data were fit by use of a modified H-model with parallel measurements of both radioassays. Variability analysis of the data based on the treatment means of the coefficients of variation across all time points and doses indicated that both studies had acceptable variability for all dose groups. The data indicate that the precision and variability of (14)C-acetate biodistribution studies are highly dependent on the total sample size of treated animals, and are comparable with more traditional studies that examine whole blood radioactivity. The (14)C-breath test was found to provide reasonable sensitivity to detect small changes in CO(2) production caused by acute or chronic drug treatment, but was not sensitive enough to measure changes in acetate-derived carbon in the liver, heart, small intestine, or kidneys.Anaphylactic shock in a patient receiving prednisone and allopurinol.
To report a case of anaphylactic shock associated with the use of allopurinol, a disorder that is difficult to manage. A 23-year-old woman with no personal or family history of asthma, with no recent personal or family history of skin or mucous membrane reactions, experienced an anaphylactic shock in the emergency department (ED) after being treated with allopurinol for hyperuricemia in the absence of acute renal insufficiency. She had recently undergone supracondylar medial humeral reduction when she was treated with allopurinol 1 month before the episode, and the allopurinol was the only medication she received the day of the reaction. She received treatment with epinephrine, hydrocortisone, and diphenhydramine. Both allopurinol and prednis

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